The City of Sausalito recognizes the following annual holidays, during which all City offices are closed except for the Sausalito Police Department and Southern Marin Fire District Station 1. Holidays that fall on a weekend or Friday will typically be observed on Thursday or Monday due to the City's four-day, 36-hour workweek for most employees.
| Date | Holiday |
| January 1 | New Year's Day |
| Third Monday in January | Martin Luther King, Jr.'s Birthday |
| Third Monday in February | Presidents' Day |
| Last Monday in May | Memorial Day |
| June 19 | Juneteenth |
| July 4 | Independence Day |
| First Monday in September | Labor Day |
| Fourth Thursday in November | Thanksgiving Holiday |
| Day After Thanksgiving | Thanksgiving Holiday |
| December 24 | Christmas Eve |
| December 25 | Christmas |